Objective To observe the effects of man-made isoleucine-lysine-valine-alanine-valine ( IKVAV ) polypeptide on the astrocyte proliferation and the gene expression of Neurocan [ core protein of chondroitin sulfate proteoglycans ( CSPGs ) ], and to explore the potential role of IKVAV in the functional recovery of the damaged spinal cord. Methods IKVAV sequence was designed and the peptide was synthesized. Astrocytes were inoculated at 1% IKVAV-coated culture plates. The histocompatibility of IKVAV polypeptide and astrocytes was observed with laser confocal microscopy ld,3d,5d,7d,9d after inoculation. The proliferation of astrocytes and Neurocan expression were detected by CCK-8 and fluorescent quantitative PCR( FQ-PCR ), respectively, at the same time points. Then the results were statistically analyzed and compared with control group. Results Compared with control group, astrocytes at IKVAV-coated culture plate were significantly reduced, but the survival rate was more than 95%. FQ-PCR results showed that Neurocan expression in IKVAV-coated astrocytes was significantly lower than that in control group. Conclusion Man-made IKVAV polypeptide can inhibit astrocyte proliferation and downregulate CSPGs expression. Consequently, it can inhibit glial scar formation and promote functional recovery of spinal cord.%目的 观察人工合成IKVAV多肽对星形胶质细胞增殖及硫酸软骨素蛋白多糖核心蛋白Neurocan基因表达的影响,并探讨IKVAV潜在的促进受损脊髓功能恢复的作用.方法 设计IKVAV序列,人工合成IKVAV多肽,将星形胶质细胞接种于1% IKVAV包被的培养板上,分别于培养1 d、3 d、5 d、7 d、9 d后在激光共聚焦显微镜下观察IKVAV多肽和星形胶质细胞的组织相容性,用CCK-8法检测星形胶质细胞增殖情况,用荧光定量PCR法检测星形胶质细胞硫酸软骨素蛋白多糖核心蛋白Neurocan的表达,并与对照组比较,进行统计学分析.结果 与对照组相比,实验组培养板上星形胶质细胞总数明显减少,但细胞存活率＞95%.荧光定量PCR结果显示实验组星形胶质细胞Neurocan的表达量较对照组显著降低.结论人工合成IKVAV多肽可抑制星形胶质细胞增殖、下调硫酸硫酸软骨素蛋白多糖的表达,从而抑制受损脊髓胶质瘢痕的形成,促进脊髓功能恢复.
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