枯草芽孢杆菌Czk1菌株对橡胶树根病菌的抑制作用及对炭疽病生防效果研究初报

摘要

[Objective]The present experiment was conducted to study the effect of Bacillus subtilis strain Czkl on controlling 13 rubber root pathogen and Colletotrichum gloeosporioides in vitro. [Method]Antagonistic activity of Czkl on 13 rubber root pathogens and C. Gloeosporioides was determined in vitro using method of agar disk diffusion and isolated leaf inoculation. [Result]Czkl had strong antagonistic effects on all the 13 rubber root pathogens and C. Gloeosporioides, and its inhibitory effects lasted until 20* generation. Czkl showed better biocontrol effects on C. Gloeosporioides, specially after its spray before disease stage. [Conclusion] Bacillus subtilis strain Czkl had strong antagonistic effects on most of the pathogenic fungi of rubber plant, viz., Ganoderma pseudoferwum, Phellinus noxius, Helicobasidium compactum, Rigidoporus ligno-sus, Sphaerostilbe repens CoIIetotrichum gloeosporioides. With its characteristic broad antibacterial spectrum, steadyness and sustainability, it has a great potential for utilization in biocontrolling rubber plant pathogens.%[目的]测定枯草芽孢杆菌Czk1菌株对橡胶树根病菌的抑菌活性及对炭疽病的生防效果,为进一步开发应用该菌株奠定基础.[方法]通过室内平板对峙和离体叶片接种的方法,测定Czk1菌株对13个橡胶树根病菌和1个炭疽病菌的拮抗活性以及对炭疽病的生防效果.[结果]Czk1菌株对13个橡胶树根病菌和1个炭疽病菌具有强烈的拮抗作用,且持续接种20代后依然具有很强的抑菌活性；Czk1菌株对橡胶树炭疽病有较好的生防效果,其中在发病前期先喷洒拮抗菌液的效果最好.[结论]枯草芽孢杆菌Czk1菌株对橡胶树红根、褐根、紫根、白根、臭根和炭疽病菌等多种致病真菌都有强烈的拮抗作用,且有抗菌谱广、持久稳定等特点,具有较高的潜在生防利用价值.