[Objective]The experiment was conducted to analyze the genetic variation amongst different mulberry diploids and their homologous tetraploids to understand the genetic mechanism of multagenesis in mulberry polyploids.[Method]The polymorphism amongst diploid and homologous tetrapioid of 8 mulberry varieties was assessed using RAPD molecular marker technique and the genetic diversity was analyzed using cluster analysis. [Results]The results showed that RAPD polymorphism between diploid and their homological tetraploid varied significantly in different mulberry varieties.Differential bands were not observed amongst diploid and their homological tetraploid in Nongsang 8 and Husang 199 varieties, while the highest RAPD polymorphism (20.41％) between diploid and its homological tetraploid was observed in Husang 32, followed by Tongxiangqing, Huangsang 37, Husang 197, Guosang 20 and Yu 71-1 ,which was 9.09, 6.06, 5.45,5.17 and 3.17％,respectively. The cluster analysis results clearly indicated the presence of two distinct clusters of the mulberry varieties used in the assessment. Husang 32 and Tongxiangqing were found in the same cluster. [Conclusion]The colchicine treatment can cause a significant genetic variations in different diploids of mulberry genotypes.%[目的]从DNA分子水平分析不同桑树二倍体及其人工诱导同源四倍体的遗传差异,为进一步探讨桑树多倍体诱变遗传规律提供理论依据.[方法]利用RAPD分子标记技术,对8个桑树二倍体品种与经秋水仙碱化学诱变获得的同源四倍体进行RAPD多态性和聚类分析.[结果]不同桑树品系二倍体间及与其人工诱导的同源四倍体间的RAPD多态性均存在明显差异.农桑8号和湖桑199号的二倍体及其同源四倍体间的扩增条带完全一致,诱导未产生变异;湖桑32号的二倍体及其同源四倍体的RAPD多态性最高,达到20.41%,桐乡青、璜桑37号、湖桑197号、国桑20号和育71-1的二倍体及其同源四倍体的RAPD多态性由高到低依次分别为9.09%、6.06%、5.45%、5.17%和3.17%.不同桑树品系间可以分为2大类,其中湖桑32号和桐乡青聚为一大类.[结论]不同桑树品系二倍体经秋水仙碱诱变后产生的遗传差异较大.
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