复硝酚钠与GA3复配对美国红栌组培苗生长的影响

摘要

[Objective] Effects of plant growth regulator combination sodium nitraphenolate+ gibberellin (GA3) at different concentrations on the growth of Cotinus coggygria were explored in order to provide technical support for shortening the culture cycle of tissue culture plants.[Method]B5 medic adding sodium nitraphenolate+GA3 of different combinations were used to culture C.coggygria.Leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number were investigated after 25 d of culture to study the effects of adding sodium nitraphenolate+GA3 on the tissue culture plants.[Result] 0.020 mg/L sodium nitraphenolate+30.0 mg/L GA3 had the optimal effects on C.coggygria tissue culture plants.Leaf number per plant,plant height,fresh weight,dry weight were significantly higher than those in control (CK) and other treatments (P＜0.05).Leaf number per plant,plant height,fresh weight,dry weight increased 83.3％、71.8％、86.6％ and 85.8％ respectively compared with CK.Rooting was also better than CK and the highest rooting rate reached 95.2％.The plants were strong with large root number,thick leaves and sound growth state.Correlation analysis indicated that there was extremely positive correlation between nitraphenolate+GA3 concentrations and leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number(P＜0.01).[Conclusion]Adding 0.020 mg/L sodium nitraphenolate+30.0 mg/L GA3 into B5 medium can improve the growth for C.coggygria tissue culture plants,and shorten the culture cycle and speed up the breeding process.%[目的]探讨生长调节剂复硝酚钠与赤霉素(GA3)复配对美国红栌组培苗生长的影响,为缩短美国红栌组培苗培养周期提供技术支持.[方法]在B5培养基中分别添加不同浓度的复硝酚钠+GA3复配液培养美国红栌组培苗,25 d后统计各处理组培苗的单株叶片数、株高、鲜重、干重、生根率及根系条数,分析复硝酚钠+GA3复配对美国红栌组培苗生长的影响.[结果]0.020 mg/L复硝酚钠+30.0 mg/L GA3复配液处理的美国红栌组培苗生长效果最佳,单株叶片数、株高、鲜重和干重均显著高于对照(CK)及其他浓度处理(P＜0.05),其中,分别比CK提高83.3％、71.8％、86.6％和85.8％,生根效果也较优,生根率最高,达95.2％,且根系条数较多,叶片肥厚、健壮,植株长势良好.相关性分析结果表明,复硝酚钠+GA3复配液浓度与美国红栌组培苗的单株叶片数、株高、鲜重、干重、根系条数和生根率呈极显著正相关(P＜0.01).[结论]在B5培养基中添加0.020 mg/L复硝酚钠+30.0 mg/L GA3复配液,可促进美国红栌组培苗生长,进而缩短其培养周期,加快种苗繁育速度.