目的:探讨助阳宁神方(ZNF)拮抗皮质酮诱导小鼠抑郁样行为的作用机制.方法:采用5-羟色氨酸(5-HTP)诱导的甩头行为模型和育亨宾毒性模型观察小鼠行为学改变;皮质酮皮下注射制备抑郁动物模型,Western blot及免疫组化检测给药4周后海马星形胶质细胞胶质纤维酸性蛋白(GFAP)表达改变.结果:5-HTP诱导的甩头行为模型中,ZNF各组甩头次数与正常组比较差异无统计学意义(P>0.05);ZNF未显著增加育亨宾毒性模型中小鼠死亡率(P>0.05);ZNF可上调模型组GFAP的表达下降.结论:ZNF抗抑郁作用可能不是通过影响单胺类神经递质产生,而可能与星形胶质细胞活性改变有关.%Objective: To explore the preventive effects of Zhuyang- ningshen formula ( ZNF) on action of depressive mice induced by corticosterone. Methods; The head-twitching test models induced by 5" hydroxytryptophane (5- HTP) and yohimbine antagonistic models were used to evaluate behavior change. Mice models were developed by subcutaneous corticosterone injection. The expressions of glial fibrillary acidic protein (GFAP) in the hippocampal tissues were decteted after drug administion on the 4th week by Western blot and immunohistochemistry. Results; The rate of head-twitching behavior was not higher in ZNF groups than that in control group(P >0. 05) ; ZNF did not enhance the yohimbine-induced lethality (P >0. 05) ; In contrast to the control group, the levels of GFAP were significantly decreased in the model group. In contrast to the model group, all dosage of ZNF water decoction could significantly improve protein levels of GFAP in the hippocampal tissues. Conclusion; The anti- depression function of ZNF can not affect monoamine neurotransmitter mechanism, butinfluence regulation of activity in astrocyte.
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