孕鼠暴露低剂量邻苯二甲酸二己酯对仔鼠脂肪分布的影响

摘要

Objective: To investigate the effect and mechanism of low dose di-2-ethylhexylphthalate ( DEHP ) exposure on fat distribution of offspring in pregnant mice .Methods: Pregnant C57BL/6J mice were divided into two groups.Mice in control group were fed with chow diet , and mice in DEHP group were fed with chow diet supplemented with 0.06%( w/w ) DEHP till their offspring was born .Nine weeks after birth , the mice were sacrificed .Epididymal and inguinal subcutaneous adipose tissues were dissected and weighted .The expression of Gpc4 and Tbx15 mRNA was measured by real-time reverse transcription polymerase chain reaction ( RT-PCR ) method.Results:The weight of epididymal adipose tissues in DEHP group was significantly higher than that in control group (P<0.05).The expression of Gpc4 mRNA in epididymal adipose tissues was significantly higher than that in subcutaneous adipose tissues ( P<0.05 ) , while the expression of Tbx 15 mRNA was significantly lower than that in subcutaneous adipose tissues ( P<0.01 ) in both control and DEHP group .Compared with control group , Gpc4 mRNA expression in epididymal adipose tissues and Tbx 15 mRNA expression in subcutaneous adipose tissues were increased ( P<0.01) .Conclusion:Low dose DEHP exposure in mice prenatal period perhaps affects fat distribution via modifying the expression of Gpc 4 and Tbx15 mRNA in visceral and subcutaneous adipose tissues.%目的：探讨孕鼠暴露低剂量邻苯二甲酸二己酯（ di-2-ethylhexylphthalate ，DEHP）对仔鼠脂肪分布的影响及机制。方法：将C57BL/6J孕鼠分为2组，对照组喂饲基础饲料，DEHP组喂饲添加质量分数为0.06％DEHP的基础饲料，至仔鼠出生。9周后，处死仔鼠，切取附睾周围和腹股沟皮下脂肪组织，称质量；实时定量RT-PCR法检测Gpc4、Tbx15 mRNA表达。结果：DEHP组仔鼠附睾脂肪组织质量显著高于对照组（ P＜0.05）；对照组和DEHP组仔鼠附睾脂肪组织Gpc4 mRNA表达均显著高于皮下脂肪组织（ P＜0.05），而Tbx15 mRNA表达则显著低于皮下脂肪组织（ P＜0.01）；相比对照组， DEHP组仔鼠附睾脂肪组织 Gpc4 mRNA及皮下脂肪组织Tbx15 mRNA表达均显著增加（ P＜0.01）。结论：胚胎期低剂量DEHP暴露可能通过改变内脏及皮下Gpc4和Tbx15的表达，影响脂肪组织的分布。