泊洛沙姆对再生丝素蛋白凝胶化结构及形态的影响

摘要

This paper studies the influences of Poloxamer on the structure and morphology of silk fibroin gelation and characterizes the effect of Poloxamer on RSF gelation through scanning electron microscope ( SEM) , infrared spectrum (FTIR) , X-ray diffraction (XRD) and thermoanalysis (TG-DTA). FTIR, XRD and TG-DTA researches show that Poloxamer significantly accelerates RSF gelation transformation after blending with RSF; meanwhile, the smaller the relative molecular mass of Poloxamer in blended solution is, the larger its blending ratio will be and the shorter its gelation time will be, but it has very small influences on the structure and morphology of hydrogel formed. SEM research shows that RSF-Poloxamer gel has a lamellar structure. The reason is that a lot of silk fibroin molecules change from random coil to β-folded structure in its process of gelation. With the increase of Poloxamer content, the lamellar structure of RSF-Poloxamer becomes more and more regular.%研究了泊洛沙姆(Poloxamer)对再生丝素蛋白(RSF)凝胶化结构及形态的影响,利用扫描电镜(SEM)、红外光谱(FTIR)、X射线衍射(XRD)和热分析(TG-DTA)表征了Poloxamer对RSF凝胶化的影响作用.FTIR、XRD和TG-DTA研究表明Poloxamer与RSF共混后极大的加速了RSF的凝胶化转变,并且随着共混溶液中Poloxamer的相对分子质量越小,所占的共混比例越大,其凝胶化时间越短,但对所形成的水凝胶的结构和形态影响很小.SEM研究表明RSF-Poloxamer凝胶为片层状结构,这是由于其凝胶化过程中大量丝素分子由无规卷曲转变为β-折叠结构所致,随着Poloxamer含量的增加,RSF-Poloxamer凝胶的片层结构变得越来越规整.