匙吻鲟表皮葡萄球菌分离鉴定与药敏试验

摘要

Objective]This study was carried out to isolation,identification and drug susceptibility test of pathogenic bacterium from Polyodon spathula,for providing a reference for prevention and treatment of hemorrhagic disease of Polyodon spathula.[Methods]Bacteria isolation from diseased paddlefish, bacteria morphological observation, physiological and biochemistry reaction by biology automatic microbiological assay system,sequencing and analysis of 16S rDNA,PCR primers specificity testis-olation,and identification of pathogenic bacterium,finally the drug susceptibility test were performed with Kirby-Bauer disc diffusion method.[Result]One strain Staphylococcus epidermidis from tissues of diseased and dying Polyodon spathula,named Pol15001,identified based on the bacterial culture,The results showed Stap hyloc oc c us e p ide rmidis were resistant to 7 kinds of drugs including selectrin trime-sulf,clindamycin,ampicillin,etc. While sensitive to ceftriaxone,mefoxin,chloroamphenicol and cef otaxime.[Conclusion]The Staphylococcus epidermidis was a new pathogenic bacteria of Polyodon spathula.Improving the aquaculture water quality and taking ceftriaxone,mefoxin,chloroamphenicol and cefo-taxime are effective preventions and controls in aquaculture.%【目的】对患病匙吻鲟进行致病菌分离鉴定和药敏试验，为匙吻鲟暴发性出血病防治提供一定的参考。【方法】从患病匙吻鲟分离细菌、形态观察、生理生化试验、16S Sr DNA和PCR引物特异性检测，对致病菌进行分离鉴定，通过K-B法进行药敏试验。【结果】从发病匙吻鲟病灶组织分离到1株致病菌菌株Pol15001，为γ溶血、革兰氏阳性球菌，生化反应结果、16S rDNA和PCR引物特异检测与表面葡萄球菌相似性均较高，药敏实验结果显示其耐药性较强，对复方新诺明、克林霉素、氨苄西林等7种受试药物耐受，仅对头孢曲松、头孢西叮、氯霉素、头孢噻肟等4种受试药物敏感。【结论】匙吻鲟为表面葡萄球菌一种新发现的宿主，生产上可通过改善水质及使用头孢曲松、头孢西叮、头孢噻肟等头孢类药物进行防治。