[目的]评价表没食子儿茶素没食子酸酯(EGCG)是否具有抗J亚型禽白血病毒(ALV-J)效应,为AVL-J防治药物的研发提供理论依据.[方法]在感染ALV-J病毒的DF-1细胞中分别添加浓度为0,5,10,20和40 μg/mL的EGCG溶液后培养的0,24,48,72和96 h,对其细胞活性、细胞中env基因表达量,禽白血病p27抗原水平,NF-κB活性以及NF-κB p50和p65蛋白表达进行了测定.[结果]EGCG对感染ALV-J病毒后DF-1的保护作用具有剂量效应, 10 μg/mL效果最好.在ALV-J侵染的DF-1细胞中添加10 μg/mL的EGCG溶液96 h后,可显著抑制由于ALV-J病毒导致的NF-κB亚基p50和p65跨膜转移,降低由于ALV-J病毒造成的细胞凋亡.[结论]EGCG通过抑制NF-κB信号的激活来提高DF-1细胞对ALV-J病毒的抵御能力,且具有显著的剂量效应,10 μg/mL为最佳添加浓度.%[Objective]To assess the antiviral effects of EGCG on ALV-J-induced cell apoptosis in vitro, and provide theoretical basis for the prevention and cure of ALV-J.[Method]DF-1 cells were treated with the EGCG concentrations of 0,5,10,20 and 40 μg/mL,respectively.Then,the cell activity,the env mRNA expression,ALV p27 antigen level,NF-κB activity,the protein expression of NF-κB p50 and p65 were examined at the 0,24,48,72 and 96 h after EGCG-treated.[Result]EGCG alleviated the ALV-J-induced apoptosis in a dose-dependent manner. High concentrations (20 and 40 μg/mL) inh-ibited the growth of DF-1 cell,and low concentration (5 μg/mL)did not suppress the ALV-J virus,so 10 μg/mL was the most appropriate concentration. After 96 h of incubation,10 μg/mL EGCG improved the ALV-J-triggered suppression of the nuclear transcription factor system by enhancing cytoplasmic NF-κB p50/p65 expression and inhibiting nuclear NF-κB p50/p65 expression,resulting in decreased cell apoptosis.[Conclusion]These results demonstrated that EGCG can increase the resistance ability to ALV-J in DF-1 cells with a dose-dependent manner via the NF-κB signaling pathway,and the optimum additive concentration was 10 μg/mL.
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