4种解释系统对HIV-1基因型耐药解释相关性评价

摘要

目的：比较HIVDB （7.0.1）、ANRS （25_09/2015）、REGA （9.1.0）和HIV GRADE （12/2015）4种解释系统对HIV-1基因型耐药结果解释的一致性。方法：选取我国3种主要HIV-1流行亚型和流行重组模式（B’、CRF01_AE和CRF07_BC），每种选取200条序列，共600条序列。分别用4种解释系统进行耐药结果分析，分为耐药（R）、中度耐药（I）和敏感（S）3个水平。对4种结果进行统计学分析。结果：4种系统基因型耐药解释结果比较相关性较高（r均＞0.57，P均＜0.01）。针对亚型来说，CRF07_BC的解释结果相关性最高，其次为B’亚型，CRF01_AE略低。结论：对于我国主要流行的HIV来说，4种解释系统对11种常用的抗病毒治疗药物耐药结果的解释具有较好的一致性。%Objective:To compare the correlation of four drug-resistance interpretation system including HIVDB, ANRS, REGA and HIV GRADE for testing HIV-1 genotype drug resistance. Methods:Trendy subtypes and restructuring model of HIV-1 including B’,CRF01_AE and CRF07_BC were selected,each genotype was 200 series,600 samples were analyzed by four drug⁃resistance interpretation system, the results were divided into three levels including resistance (R), possible drug⁃resistance (I) and susceptible (S). Results:Four drug⁃resistance interpretation system had a high correlation in genotypic drug⁃resistance consequence (rs>0.57, P<0.01), CRF07_BC had the highest correlation, followed by subtype B, CRF01_AE was slightly lower.Conclusion:Four drug⁃resistance interpretation system has a high correlation in analyzing antiviral drug resistance for major epidemic of HIV in China.