乳腺癌干细胞标志物CD44/CD24与ALDH1的相关性分析

摘要

目的 分析CD44/CD24与乙醛脱氢酶1 (ALDH1) 两种乳腺癌干细胞标记物表达的阳性细胞比率及其相关性.方法收集培养4例乳腺癌转移胸腔积液和8株乳腺癌细胞系, 进行CD44/CD24与ALDH1的标记染色, 应用流式细胞术检测CD44+CD24-与ALDH1+细胞亚群的比例, 并分析两种标记物之间的相关性.结果 8株乳腺癌细胞系CD44+CD24-细胞亚群比例仅2株高达85％以上, 其余均在5％以下; ALDH1+细胞亚群比例总体较低, 在0. 98％-25. 2％之间.4例胸腔积液中, CD44+CD24-细胞亚群比例较高, ALDH1+细胞亚群则比例波动较大.乳腺癌细胞系CD44+CD24-与ALDH1+细胞亚群比例的相关性分析显示, 两种标记物无相关性 (r=-0. 168 3, P=0. 383) .对ALDH1+、ALDH1-与未标记细胞中CD44+CD24-细胞亚群比例分别进行比较分析, 未见差异.结论 CD44/CD24与ALDH1两种乳腺癌标记物在乳腺癌细胞中的表达是独立的, 二者没有相关性.%Objective To analyze the positive ratio of breast cancer stem cells marker CD44/CD24 and acetaldehyde dehydrogenase 1 (ALDH1) and their correlation. Methods Four cases of breast cancer metastatic pleural effusion and eight breast cancer cell lines were collected and cultured, and then stained with two breast cancer stem cell markers (CD44/CD24 and ALDH1). The ratios of CD44+CD24-and ALDH1+subpopulation were detected by flow cytometry, and the correlation between two markers was analyzed.Results The ratios of CD44+CD24-subpopulation in 2 of 8 breast cancer cell lines were higher than 85％, while the ratios in the rest cell lines were below 5％. The ratio of ALDH1+subpopulation was relatively low, from 0. 98％ to 25. 2％. In 4 cases of pleural effusion, the proportion of CD44+CD24-subpopulation was high in total, however, the ratio of ALDH1+subpopulation fluctuated greatly.And there was no significant correlation between the expression of CD44/CD24 and ALDH1 (r =-0. 168 3, P = 0. 383). Furthermore, the ratio of CD44+CD24-subpopulation in ALDH1+, ALDH1-and unlabeled cells showed no difference. Conclusion The expression of CD44/CD24 and ALDH1 in breast cancer cell is independent and there is no correlation between them.