胰岛素样生长因子Ⅰ的抗心肌细胞凋亡作用

摘要

The effects of insulin-like growth factor-Ⅰ (IGF-Ⅰ) on cardiomyocyte apoptosis induced by hypoxia in cultured neonatal rat cardiac myocytes were investigated.Primary neonatal rat cardiomyocytes were cul tured in 95% N2－5% CO2 to imitate in vivo hypoxic condition.DNA gel ele ctrophoresis showed DNA laddering in an ischemic duration-dependent manner.The hypoxia-induced cardiomyocyte apoptosis was also evidenced by flow cytometry as say.DNA gel electrophoresis showed that IGF－Ⅰ in a dose range of 10-9～1 0-7mol/L could significantly prevent the hypoxia-induced cardiomyocyte ap optosis.The protective effects of IGF－I against hypoxia-induced apoptosis coul d also be verified by flow cytometry assay.%研究了缺氧条件下引起培养的乳鼠心肌细胞凋亡及IG F－Ⅰ的抗凋亡作用。将原代培养的心肌细胞置于95% N2－5%CO2缺氧环境下，DNA凝胶 电泳及流式细胞计数检测显示细胞凋亡与缺氧呈时间依赖性。在缺氧前加入10-9～10 -7mol/L IGF－Ⅰ能有效地抵抗缺氧引起的心肌细胞凋亡。