特发性矮小患儿IGF-1R、IGFALS蛋白的表达

摘要

目的 检测特发性矮小患儿血清IGF-IR、IGFALS蛋白的水平,探讨其在特发性矮小(ISS)患儿生长发育中的作用.方法 收集就诊的特发性矮小儿童26例作为实验组(ISS组),抽取10例年龄、性别相匹配的正常身高儿童作为对照组.对他们进行全面的病史采集和体格检查,行生长激素激发试验,检测IGF-1和IGFBP3,并检测血清IGF-1R、IGFALS蛋白表达水平,比较两组中IGF-1R、IGFALS蛋白表达是否有差异,并分析IGF-1R、IGFALS蛋白表达与各项临床指标的相关性. 结果 ①ISS组血清IGF-1R表达水平明显低于对照组,差异具有统计学意义(P＜0.05),ISS组和正常对照组血清IGFALS蛋白水平差异无统计学意义(P≥0.05).②ISS组血清IGFALS蛋白及IGF-1R蛋白表达量均与身高差呈负相关(P＜0.05),IGF-1R蛋白表达与年龄呈正相关(P＜0.05),IGFALS蛋白表达与年龄无相关性(P≥0.05). 结论 ①IGF-1R和IGFALS蛋白表达水平越低可能使ISS患儿身高差更大,患儿越矮小.②IGF-1R缺乏可能是部分特发性矮小患儿的原因.%Objective To investigate the levels of serum IGF-1R and IGFALS proteins in children with idiopathic short stature.Methods A total of 20 children with idiopathic short stature (ISS group) were enrolled,and l0 age-and sex-matched normal height children were selected as control group.The data,including a comprehensive medical history,physical examination,growth hormone stimulation test,were collected.The IGF-1 and IGFBP3 levels were detected,and the serum IGF-1R and IGFALS levels were measured and compared between the two groups.The correlation between the expressions of IGF-1R and IGFALS proteins and the clinical parameters was analyzed.Results ①The serum IGF-1R protein expression levels in ISS group was significantly lower than that in control group(P ＜ 0.05),while the serum IGFALS protein level showed not statistically significant difference between ISS group and control group.②For the ISS patients,the expression of IGFALS protein and IGF-1R protein was negatively correlated with the difference in height(P ＜ 0.05),the expression of IGF-1R was positively related to age,but the expression of IGFALS protein was not correlated with age.Conclusion ①For the ISS patients,the expression of IGF-1R and IGFALS proteins is related to the difference in height.②IGF-1R deficiency may be the cause of some idiopathic short stature in children.