Inositol hexakisphosphate (IP6) and the mixed solution of zinc oxide nanoparticles and hemoglobin(Hb) were alternately modified onto the surface of a glassy carbon electrode ( GCE) through layer-by-layer self-assembly technique for constructing | Hb-ZnO/IP6 16/GCE. | Hb-ZnO/IP6 16/GCE was characterized with cyclic voltammetry ( CV) .electrochemical impedance spec-troscopy (EIS) and field emission scanning electron microscopy (FESEM). Experimental results showed that the | Hb-ZnO/ IP6 (6/GCE exhibited three-dimensional porous structure and realized the direct electron transfer between Hb and electrode surface, j Hb-ZnO/IP6 |6/GCE displayed excellent electrocatalytic activity for the reduction of H2O2 with a linear response range from 2.00 x 10"6-l. 12 x 10"4 mol ■ L"1 and a detection limit of 1. 40 x 10"6mol ? L"1 (at the ration of signal to noise, S/N =3). The Michaelis-Menten constant K M*pp was estimated to be 5.1 mmol ■ L"1.%将纳米氧化锌与血红蛋白(Hb)的混合液,通过肌醇六磷酸酯(IP6)层层自组装于玻碳电极(GCE)表面,制备了{Hb - ZnO/IP6}6修饰电极.用循环伏安法、电化学阻抗光谱和场发射扫描电镜对{ Hb - ZnO/IP6}6膜进行了表征.实验结果表明:{Hb - ZnO/IP6}6/GCE呈现层状三维多孔结构,实现了血红蛋白和电极表面的直接电子转移.该修饰电极对过氧化氢有很好的电催化活性,线性范围为2.00×10-6～ 1.12×10 -4mol·L-1,最低检测限为1.40 × 10-6mol·L-1(信噪比S/N =3),米氏常数为5.1 mmol · L-1.
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