生物体系中有机小分子构建的pH值检测荧光探针研究进展

摘要

As traditional pH meters cannot work well for the real-time determination and quantitative detection of pH value chan-ges in microenvironments ( such as subcellular organelles ) and complex biological systems ,molecular pH-sensitive probes for mo-nitoring pH changes in complicated and changeable biological systems are urgently needed .This review focuses on the current pro-gress in small molecular fluorescence probes for the detection of pH value changes in biological systems ( mainly in monitoring the pH fluctuation in the live cell ) .Future development of fluorescent probes for pH value detection in the biological systems was also discussed .%由于传统的pH计不能很好地应用在微环境(如亚细胞器)和复杂生物体系中的pH值动态和精确定量检测,因此迫切需要研究可监测复杂多变生物体系中pH值变化且具有高灵敏度的pH值响应分子探针.就近年来用于生物体系(主要是活细胞内)pH值检测的小分子荧光探针作简要综述,同时对目前基于有机小分子构建的荧光探针用于生物体系pH值检测研究及其未来的发展趋势进行展望.