Objective To clarify the polo-like kinase1 (PLK1) expression in human gastric cancer tissue and its clinicopathological sig-nificance in gastric carcinoma. Methods PLK1 expression in 60 cancer tissues and their corresponding noncancerous tissues from gas-tric cancer patients was measured by both real-time quantitative RT-PCR and western blot assay. Immunohistochemistry was used to detectPLK1 protein expression in eighty-nine paraffin-embedded samples. Results The PLK1 mRNA and protein expression level in the 60fresh cancer tissues was significantly higher than that in noncancerous tissues (P<0.0001,P=0.031 respectively). In paraffin-embed-ded samples, apart from its increased expression level, PLK1 was found to be in both cytoplasm and nucleus, double-site location onlyoccurred in poor-differentiated cancer, PLK1 expression intensity was associated with tumor differentiation (P=0.03), invasion(P=0.032), TNM stage (P=0.019), ki67 expression (P=0.011). The patients with negative PLK1 expression had better survivalrate than that with positive PLK1 expression (P=0.0292). Conclusion PLK1 may have clinicopathological value in tumor diagnosis,and may become another new bio-marker and therapeutic target for gastric cancer.
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