Objective Possible differences were analyzed between histopathological and molecular biologicalfindings in laryngeal cancer tissues and its adjacent nomal tissues. Methods Laryngeal carcinoma tissues from20 patients were analyzed by PCR-SSCP. Results P53 gene mutations were detected in 25% (5 out of 20) of thecarcinomas. In these patients P53 mutations were detected in histopathologically nomal tissue both within 0.5 cmand more than 0.5cm distant from the nearest cancerous tissue. Conclusion Since the P53 mutation was commonin both cancer tissue the gene mutation may plan an.important role in laryngeal carcinogenesis. Radical excision ofthe tumour is defined by histopathological limits, but"normal" tissue may nevertheless contain the gene mutation,giving rise to the danger of further cancer development. We suggest that the determination of the surgical marginshould be based on a combination of histopathology and molecular biological findings.
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