大鼠肝微粒体匀浆中阿司匹林酯酶热稳定性的研究

摘要

Objective To study the thermal stability of aspirin esterase in rat liver microsomes .Methods Culture of homogenate of rat liver microsomes was cultured as the source of enzyme in vitro after being at 30℃ ,40℃and 50℃ .The concentrations of aspirin and salicylic acid were determined by RP- HPLC and CSA/(CASA+CSA) was used to reflect the activity of aspirin esterase .Results The activity of aspirin es-terase in rat liver microsomes declined to 15% at 50 ℃ and remained for 10 minutes ,declined to 20% at 40℃ and remained for 60 minutes ,and declined to 60% at 30℃and remained for 60 minutes .Conclusion Aspirin esterase in rat liver microsomes shows thermal instability and needs to be preserved under low tem-perature .%目的：本实验探究大鼠肝微粒体匀浆中阿司匹林酯酶的热稳定性。方法将大鼠肝微粒体匀浆分别在30、40、50℃的条件下处理后作为酶来源进行体外培养，以RP-HPLC法测定培养体系中阿司匹林（ASA）和水杨酸（SA）的浓度，以CSA/（CASA＋ CSA）表示阿司匹林酯酶的活性。结果大鼠肝微粒体匀浆中阿司匹林酯酶在50℃下保存10min活性基本消失，仅剩15％；在40℃的条件下，保存60min后酶活性降低至20％，在30℃保存60min大鼠肝微粒体匀浆中阿司匹林酯酶活性约为60％。结论大鼠肝微粒体匀浆中阿司匹林酯酶对热不稳定，需低温保存。