在不同温度下,用浓度分别为10μmol/L、20 μmol/L、40μmol/L的血卟啉孵育L1210和S180肿瘤细胞,通过荧光分光光度法检测血卟啉在L1210及S180肿瘤细胞内的富集以及温度对其的影响,激光共聚焦扫描显微镜观察血卟啉的亚细胞定位.结果表明,随着孵育浓度的增加和孵育时间的延长,肿瘤细胞对血卟啉的吸收也相应增加,60～120 min细胞内血卟啉含量最高;不同温度也会影响细胞对血卟啉的吸收,37C为最适孵育温度.%It have been investigated that hematoporphyrins were retained in L1210 and S180,respeceively. The cells were incubated with hematoporphyrin ( HpD) at 10μmol/L,20μmol/L,40μmol/L concentrations and different temperatures. The intracellular HpD concentration and the effect imposed by temperature were measured by the fluorescence photometer, and laser scanning confocal microscope was used to study the subcellular location pattern of HpD. The results showed that the intracellular concentration of HpD increased with the increasing of HpD and incubated time,the intracellular concentration was highest in 60～120 min; the absorption of HpD could also be influenced by temperature, and 37 ℃ is the most suitable incubation temperature.
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