Objective:To analyze the outcomes of artificial insemination by frozen-thawed donor's semen(AID)and its affecting factors.Methods:Retrospective analysis of the results of 412 AID cycles performed in 173 couples be-tween February 2002 and December 2003 was presented,to evaluate the influence of female age,methods of insemination,therapeutic regime,post-thaw semen motility and number of treatment cycles on the fecundity of women undergoing AID.Results.Overall pregnancy rate of 31.6%(130/412)and delivery rate of 27.2%(112/412)per cycle and cumulative pregnancy rate of 72.3%(125/173)were achieved,with abortion rate of 13.9%(18/130)and multiple pregnancy rate of 2.68%.In 125 pregnant women,inseminations were performed 1-5 cycles,and 89.2% pregnant women conceived within three treatment cycles with twice AID in each cycle.Some factors such as ovarian stimulation(OS),female age(under 38 years),methods of insemination and luteal phase support bear no significant relations to preg-nancy rate,but the motility of post-thaw semen was significantly related to pregnancy rate.A-mong 115 neonates with weight 2,750~5,000 g,one was found congenital ventricular septal de-fect.Conclusions:Insemination with frozen-thawed semen is safe and effective.In women less than 38 years with normal reproductive function,satisfactory pregnancy rate could be achieved when AID was performed twice per cycle before and after ovulation within 4 consecutive sponta-neous cycles.
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