甲氨蝶呤对脂多糖诱导大鼠滑膜细胞株RSC-364分泌IL-17和IL-18的影响

摘要

Objective To investigate the effect of methotrexate ( MTX ) on expression of IL-17 and IL-18 in the rat synovial cell line RSC-364 stimulated with lipopolysaccharide ( LPS ) and to illuminate the mechanism of MTX in the treatment of rheumatoid arthritis ( RA ). Methods The expression of IL-17 and IL-18 in the rat synvoial cell line RSG-364 stimulated with LPS induced by different doses of MTX were detected by enzyme-linked immunosorbent assays. Results The expression of IL-17 and IL-18 in the rat synvoial cell line RSC-364 stimulated with LPS induced by MTX were obviously suppressed. Conclusion IL-17 and IL-18 in the rat synovial cell line RSC-364 stimulated with LPS were inhibited by MTX, which might be the potential mechanism in the treatment of RA.%目的:观察甲氨蝶呤对脂多糖诱导大鼠滑膜细胞株RSC-364分泌IL-17和IL-18表达的影响,进一步探讨甲氨蝶呤治疗类风湿性关节炎(RA)的作用机理.方法:运用ELISA检测不同浓度的甲氨蝶呤对脂多糖诱导大鼠滑膜细胞株RSC-364细胞上清液中的IL-17和-IL-18的含量.结果:甲氨蝶呤明显抑制脂多糖诱导大鼠滑膜细胞株RSC-364细胞分泌炎性因子IL-17和IL-18的含量.结论:甲氨蝶呤抑制脂多糖诱导大鼠滑膜细胞株RSC-364分泌IL-17和IL-18,这可能是其治疗RA的重要的作用机制之一.