Objective In this study, changes of plasma beta-defensin-3 level were determined in patients with bone fracture operative site infections and its correlation with disease was evaluated. Methods 18 patients with bone fracture operative site infections and 103 patients without those were collected. Plasma beta-defensin-3 concentration was measured by ELISA. The correlation with disease was analyzed statistically. Results The plasma beta-defensin-3 level (64.1 26. 3 pg/ml) in patients with bone fracture operative site infections was significantly lower than that (134.6 50. 2 pg/ml) in patients without those using analysis of covariance ( F =7.254, P<0.001). On a multivariate logistic regression, plasma beta-defensin-3 level (OR=0.681, 95%CI=O.391~0.864, P<0.05) was an independent variable predicting operative site infection. A receiver operating characteristic curve identified that a plasma beta-defensin-3 level < 95. 8 pg/ml predicted operative site infection with 77. 8% sensitivity and 82. 5% specificity separately (area under curve = 0.842, 95% Cl = 0. 795 ~ 0. 899, P < 0.001), Conclusion Decreased beta-defensin-3 is found after bone fracture operative site infection and low beta-defensin-3 maybe independently associated with bone fracture operative site infection.%目的:揭示骨折手术部位感染患者血浆β-防御素-3浓度的变化,探讨其与骨折手术部位感染危险性的相关性.方法:收集骨折手术部位感染患者18例和非感染患者103例.ELISA检测血浆β-防御素-3浓度.统计分析其与骨折手术部位感染危险性的相关性.结果:协方差分析显示,骨折手术部位感染患者血浆β-防御素-3浓度(64.1±26.3)pg/ml显著地低于非感染患者(134.6±50.2)pg/ml(F=7.254,P＜0.001).Logistic回归分析显示,血浆β-防御素-3浓度(OR=0.681,95％CI=0.391～0.864,P＜0.05)是骨折手术部位感染的独立危险因素.ROC曲线分析显示,血浆β-防御素-3浓度预测骨折手术部位感染有显著预测价值(曲线下面积=0.842,95％CI=0.795～0.899,P＜0.001),且判定血浆β-防御素-3浓度＜95.8pg/ml,对预测骨折手术部位感染有77.8％的灵敏度和82.5％的特异度.结论:骨折手术部位感染患者血浆β-防御素-3水平降低,可能是骨折手术部位感染的危险因素.
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