慢性乙肝、肝硬化和肝癌患者Th1/Th2细胞因子检测及临床应用

摘要

Objective To detect plasma Thl/Th2 cytokines in CHB, liver cirrhosis and HCC patients with CBA methods, to ex-plore the associated clinical value. Methods CBA method was used to detect Thl cytokines(TNF-α,IFN-γ,IL-2) , Th2 cytokines (IL-4,IL-5,IL-10) in 71 CHB patients, 33 HBV positive liver cirrhosis patients and 25 HBV positive HCC patients. Results The expression of thl cytokines (TNF-α,IFN-γ,IL-2) and IL-5 were significantly higher in HCC patients than in CHB and liver cirrhosis panents(P ＜0.01～0.05) , the expression of IL-4 and IL-10 showed no difference in three groups of patients. Conclusion High expression of Th1 cytokines in malignant hepatoma patients indicates it is high immununological response in these patients. Detection of Th1/Th2 cytokines may be a new clinical predictive indicator for the progress of chronic liver disease.%目的:应用流式微球技术(cytometric bead array,CBA)技术检测慢性乙肝、肝硬化及肝细胞性肝癌(hepatic cellular cancer,HCC)患者血浆中Th1/Th2细胞因子水平,探讨其临床应用.方法:选择慢性HBV感染者71例,HBV阳性肝硬化患者33例,HBV阳性肝癌患者25例,用CBA方法检测其血清中Th1类细胞因子TNF-α、IFN-γ、IL-2,Th2类细胞因子IL-4、IL-5、IL-10的表达情况.结果:肝癌患者血清中Th1类细胞因子TNF-α、IFN-γ、IL-2,Th2类细胞因子IL-5表达显著高于慢性乙肝和肝硬化患者,而IL-4、IL-10在三组患者中的表达无显著性差异.结论:肝癌患者机体强势分泌Th1类细胞因子,处于高免疫反应状态.检测乙肝患者Th1/Th2类细胞因子对于肝病慢性化的进展成为一项新的临床预测指标.