Hsa－miR－133 a在非小细胞肺癌中的表达及其与临床病理联系

摘要

Objective This article was to investigate the correlation between Hsa -miR -133a expression and clinic -pathological characteristics, which would be useful evidence to predict the progression degree of NSCLC.Methods The RT-qPCRmethod was used to measure the expression of miR-133ain non-small cell lung carcinoma tissue and pericarcinomatous tissue.The general features of patients with NSCLC( such as age, gender) and clinic-pathological data ( such as clinical stage, lymph node metastasis, distant metastasis, pathology classification, histological differentiation ) were collected.The relationship between the expression of miR-133a and clinic-pathological characteristics was explored by Kaplan-Meier test.Results The expression of miR-133a decreased about 6.571 folds ( P=0.000) in NSCLCs compared with matched pericarcinomatous tissue.Compared with no lymph node metastasis of lung cancer, expression of miR-133a in lung cancer with lymph node metastasis decreased (P=0.010), and lower average about 1.381 folds.The expression of miR-133a statistically associated with lymphnode metastasis, clinic stage and pathologic differentiated degree in NSCLC.Correlations of miR-133a were not observed in age, gender, pathological classification and tumor size. Conclusions Compared with pericarcinomatous tissues, the expression of miR-133a downregulated in non-small cell lung carcinoma tissue.The Expression of miR -133a was statistically associated with lymph node metastasis, clinical stage and pathologic differentiation in lung cancer.%目的：探讨Hsa－miR－133a在非小细胞肺癌（ NSCLC）中的表达及其与NSCLC临床病理特征之间的相关性，为Hsa－miR－133a预测肺癌发生风险和进展程度提供客观依据。方法采用实时荧光定量PCR检查50例NSCLC及癌旁组织中Hsa－miR－133a表达情况；收集患者一般资料（如年龄、性别）和临床病理资料（临床分期、淋巴结转移、远处转移、病理类型、组织分化程度），通过卡方检验分析Hsa－miR－133a与NSCLC患者临床病理特征之间的相关性。结果与癌旁肺组织相比较，50例NSCLC组织中，Hsa－miR－133a表达下降（P＜0．05），其表达的平均水平降低约6．571倍（P＝0．000）。与不伴淋巴结转移的肺癌组织相比，伴有淋巴结转移的肺癌组织中，Hsa－miR－133a表达下降（P＜0．05），其表达的平均水平降低约1．381倍（P＝0．010）。 Hsa－miR－133a表达情况与病理分级、临床分期和淋巴结转移有关（P＜0．05），与年龄、性别、病理类型以及肿瘤大小无关（P＞0．05）。结论 Hsa －miR－133a 在NSCLC组织中表达下调与NSCLC淋巴结转移、远处转移和病理分期有关。