采用灭活的鱼肠道弧菌作为抗原,制备兔抗血清,建立了鱼肠道弧菌的间接荧光抗体检测技术( indirect immunofluorescence assay test,IFAT)、Western - blotting免疫印迹检测技术.IFAT结果显示阳性信号覆盖整个菌体,明亮,清晰,荧光信号呈长弧形且两端钝圆,与鱼肠道弧菌形状一致.Western - blotting结果显示共10条蛋白带发生免疫反应,其中6条蛋白带结果较清晰,显示出较强的特异性免疫反应,阴性对照组无条带.IFAT和Western -blotting结果说明所建立检测方法能够准确、快速的检测出鱼肠道弧菌.%In this paper, indirect immunofluorescence assay test (IFAT) and Western -blotting for rapid diagnosis of Vibrio ichthyoenteri have been developed. Antiserum specific for the Vibrio ichthyoenteri was produced from New Zealand white rabbit and used in IFAT and Western - blotting. The consequent of IFAT showed that light and clear positive signals covered the whole bacteria with the shape of long arc and roundness which fitted with Vibrio ichthyoenteri. Western blotting analysis revealed ten antigenic protein bands. Six major antigens with apparent molecular masses of approximately 88, 79, 72, 61, 42 and 18 kDa as well as other four less immunoractive bands of approximately 102, 36, 34 and 29 kDa were distinguished. This study will facilitate the development of rapid diagnostic techniques for Vibrio ichthyoenteri.
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