梨矮化砧优系'青砧D1'叶片离体培养再生体系的建立

摘要

"青砧D1"是青岛农业大学选育的一个梨矮化砧优系.本试验研究了不同浓度的TDZ、6-BA、IBA及IAA配比对"青砧D1"叶片离体再生培养的影响,从而为该砧木的快速繁殖和应用推广奠定基础.结果表明:MS培养基中附加适宜浓度和配比的6-BA与IBA能提高初代培养外植体的成活率,以6-BA 1.0 mg·L-1与IBA 0.1 mg·L-1浓度配比的外植体成活率最高,达86.3%.NN69培养基中附加不同浓度的TDZ与IBA或IAA能有效地诱导叶片外植体不定芽再生,以TDZ和IBA配合诱导产生不定芽的外植体比例高,在TDZ 4.0 mg·L-1与IBA 0.3 mg·L-1配比组合下可达到100%,但平均外植体再生芽数为3个,低于TDZ与IAA配比组合(3.9个/外植体).在所试的不同浓度TDZ与IAA配比组合中,外植体不定芽诱导率最高仅为68%.MS培养基中附加6-BA 1.0 mg·L-1和IBA 0.2 mg·L-1时芽增殖系数最高,达4.42.在1/2 MS培养基中附加IBA 0.3 mg·L-1时生根率最高,达87.3%,移栽成活率达95%.%"Qing zhen D1" was a excellent pear dwarfing rootstock line selected by Qingdao Agricultural University.In this experiment, the effects of different combinations and concentrations of cytokinin (TDZ, 6-BA) and auxin (IBA, IAA) on the regeneration were examined in"Qing zhen D1", laying the foundation for rapid propagation, application and promotion of this rootstock.The results showed that MS supplemented with appropriate concentrations of 6-BA and IBA could improve the survival rate of primary cultured explants, up to 86.3% with 6-BA 1.0 mg·L-1 + IBA 0.1 mg·L-1.Combination of TDZ and IBA or IAA on NN69 medium could effectively induce the adventitious bud.And the relatively high regeneration rate was induced by TDZ and IBA, could up to 100%, with TDZ 4.0 mg·L-1 + IBA 0.3 mg·L-1.But the average induced adventitious bud number was just 3 per explant, which was lower than that of TDZ and IAA combination (3.9/explant).Among the different concentration combinations of TDZ and IAA tested, the hightest induction rate of adventitious bud was only 68%.MS medium supplemented with 6-BA 1.0 mg·L-1 and IBA 0.2 mg·L-1 could induce the highest bud multiplication coefficient, up to 4.42.The hightest rooting rate was 87.3% and the survival rate of transplanting was 95% when 0.3 mg·L-1 IBA was added in 1/2 MS medium.