目的:探讨Garcinol对二甲基苯并蒽(7,12-dimethylbenz anthracene,DMBA)诱发的金黄地鼠口腔癌的化学预防作用.方法:30只金黄地鼠分3组:阴性对照组(6只)不涂药;阳性对照组(12只)涂0.5%DMBA于左侧颊囊,每周3次,共涂3周;Garcinol组(12只)前3周处理同阳性对照组,3周后换涂5 mmol/L Garcinol,每周3次,涂1周.第4周末实验结束处死所有地鼠,处死前2 h腹腔注射50 mg/kg的5-溴脱氧尿嘧啶(5-bromodeoxyuridine,BrdU).取左侧颊囊行组织病理学观察和BrdU免疫组化染色.结果:地鼠颊囊涂Garcinol 1周后,炎症细胞数/mm2由阳性对照组的158.65±26.51减少到55.03±22.80(P<0.01).单纯增生的病灶数由4.60±1.42减少到1.25±0.82(P<0.05).异常增生的病灶数由3.47±1.12减少到1.13±0.67(P<0.05).BrdU增殖指数由6.57±2.03减少到2.17±0.53(P<0.01).结论:Garcinol对DMBA诱发的金黄地鼠口腔癌前病变具有一定的化学预防作用,其机制可能与抑制炎症和细胞增殖有关.%Objective: To study the preventive effect of Garcinol on 7,12-dimethylbenz anthracene (DMBA) induced oral precancerous lesion in Golden Syrian hamsters. Methods: 30 hamsters were divided into three groups. 6 hamsters in health group were not treated, 24 were treated with 0.5％ DMBA solution topically in the left cheek pouch three times per week for three consecutive weeks. After the last DMBA treatment ,the hamsters were divided into two groups at random. 12 animals in control group were not treated, 12 in treatment group received 5 mmol/L Garcinol three times within the following week. 4 weeks after the start of the experiment the animals were injected with 5-bromodeoxyuridine (BrdU) at 50 mg/kg and then sacrificed. The cheek pouch samples were collected for histopathology and BrdU immunohistochemisty. Results: Garcinol significantly decreased the number of inflammatory cells/mm2 in the lesion tissue from 158.65 ± 26. 51 to 52.25 ± 18.53 ( P ＜ 0.01 ). The incidence of hyperplasia was decreased from 4.60 ± 1.42 to 1.63 ± 0. 65.The incidence of dysplasia was decreased from 3.47 ± 1.12 to 1.82 ± 0.77. Garcinol decreased the BrdU-index from 6.57 ± 2.03 to 2.17 ± 0.53 (P ＜ 0. 01 ). Conclusion: Garcinol has some effect on the prevention of DMBA-induced oral precancerous lesion and such prevention may be related to the effect of anti-inflammation and the suppression of cell proliferation.
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