Objective: To determine the microbial indicators for prediclion and assessment of the treatment outcome by investigating the relationship between the changes of the levels of Porphyromonas gingivalis (P. G) , Tannerella forsythia (T.f) , Prevolella intermedia (P. I) , Aggregatibacter actinomycelemcomitans(△.a) and Treponema denticolai T. D) and the clinical parameters following scaling and root planning (SRP) in patients with chronic periodontitis. Methods, 20 patients (12 female and 8 male) with chronic periodontitis were enrolled in this study. Clinical parameters including PPD, CAL and BOP were monitored and subgingival plaque samples were mi-crobiologicaly examined before and 3 months after therapy. All subjects received full mouth SRP. Semi-quantification of the five microorganisms in the subgingival plaque were determined by polymerase chain reaction ( PCR) and reverse hybridization assay. Results, After therapy, the levels of P. G, T.f and T. D of the sites with PPD reduction of ≥2 mm decreased (P <0. 05) . The mean PPD reduction of the P. G-undecreased sites (1. 16 ±0. 83) mm and the T. F-undecreased sitesi 1.02 ±0.94) was respectively less than the P. G- decreased sites (2. 00 ± 1. 22) mm and the T. /-decreased sites (2. 04 ± 1.17) mm(P<0.05). The BOP positive ratio (%) at the T. F-decreased sites(38.5%) and T. D-decreased sites(20%) were significantly less than that at T. F-undecreased sites (71.4%) and T. D-undecreased sites (60%) . Conclusion, The control of the levels of P. G, T.f and T. D is important in the improvement of PPD and BOP. P. G, T.f and T. D may be microbial indicators for the prediction and assessment of SRP outcome.%目的:观察洁刮治(SRP)后慢性牙周炎患者龈下牙龈卟啉单胞菌(P.g),福赛氏类杆菌(T.f),中间普氏菌(P.i),伴放线放线杆菌(A.a)和齿垢密螺旋体(T.d)等5种可疑致病菌的变化与临床指标改善的关系.方法:对符合纳入标准的20例慢性牙周炎患者进行SRP治疗,记录治疗前及治疗后3个月牙周探诊深度、临床附着丧失和探诊出血的变化,半定量检测5种龈下牙周可疑致病微生物.结果:治疗后PPD减少≥2mm的位点、P.g、T.f和T.d的检出量都有显著减少(P＜0.05).治疗后PPD的改善在P.g检出量减少的位点[(2.00±1.22)mm]和T.f检出量减少的位点[(2.04±1.17)mm]分别显著高于其检出量未减少的位点[(1.16±0.83)mm和(1.20±0.94)mm](P＜0.05).治疗后BOP阳性率在T.f检出量减少的位点(38.5%)和T.d检出量减少的位点(20%)分别显著低于其检出量未减少的位点(71.4%和60%)(P＜0.05).P.i和A.a治疗前后检出量的变化与临床指标的改善间无明显关系.结论:有效控制P.g、T.f和T.d的量与PPD和BOP的改善密切相关,提示P.g,T.f和T.d可以作为评估SRP治疗效果的标志性微生物.
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