钾离子通道阻滞剂4-AP 对人舌鳞癌 Tca8113细胞增殖的影响

摘要

Objective:To study the effects of a potassium channel blocker 4-Amino pyridine(4-AP)on the proliferation of human tongue squamous cell carcinoma Tca8113 cells.Methods:CCK-8 assay was used to detect the proliferation of Tca8113 cells cultured with 4-AP at the concentration of 1,5,10,20,50 and 100 mmol/L for 12,24 and 48 h respectively,the cell proliferation inhibition rate was calculated,the cell cycle distribution was examined by flow cytometry.Data were analyzed by SPSS19.0 software.Results:With the increase of 4-AP concentration and culture time,cells showed some morphologic changes.4-AP at 5 -100 mmol/L dose and time dependently inhibited the proliferation,with 24 h exposure dose dependenty decreased S-phase population and increased G0 /G1 phase population of Tca8113 cells.Conclusion:4-AP may inhibit Tca8113 cell proliferation by regulation of the cell cycle distribution.%目的：检测4-氨基啶（4-AP）对 Tca8113细胞株增殖的影响。方法：采用 CCK-8法检测不同浓度4-AP 作用于 Tca8113细胞株12、24、48 h 后对细胞的增殖抑制率；流式细胞术（FCM）检测各组细胞周期变化。采用 SPSS19．0软件对数据进行统计学分析。结果：随着4-AP 浓度的增加和干预时间的延长，Tca8113细胞形态发生改变。在浓度5～100 mmol／L 范围内作用于细胞12～48 h，4-AP 浓度和时间依赖性地抑制了 Tca8113细胞的增殖（P ＜0．01）。不同浓度4-AP 作用于 Tca8113细胞株24 h后，G0／G1期的细胞所占的比例显著升高（P ＜0．01）；S 期细胞所占的比例显著降低（P ＜0．01）；4-AP 阻滞 Tca8113细胞株于G0／G1期。结论：4-AP 可能通过调控细胞周期抑制 Tca8113细胞株的增殖对细胞株分布有影响。