三维重建观察四氯二苯并二恶英对胎鼠腭器官发育的影响

摘要

目的:三维重建观察四氯二苯并二恶英(tetrachlorodibenzo-p-dioxin,TCDD)对胎鼠腭器官发育的影响.方法:对妊娠12.5 d昆明鼠进行40 μg/kg TCDD灌胃处理作为实验组,等剂量的玉米油处理作为对照组.妊娠13.5、14.5、15.5 d时,取出胎鼠头部固定,制备腭器官石蜡连续切片,苏木精-伊红染色,采集腭器官的图像用Photoshop处理、3D-DOCTOR软件对腭器官进行三维重建.结果:3D-DOCTOR三维重建显示,对照组腭器官从舌两侧移至舌上方,逐渐靠拢并融合;实验组腭器官从舌两侧延迟于对照组移至舌上方渐靠拢,但并未融合,形成腭裂.结论:3D-DOCTOR重建可观察腭器官发育过程和TCDD作用于孕鼠导致胎鼠腭裂形成过程.%Objective:To study the feasibility of 3-D reconstruction model in the observation of tetrachlorodibenzo-p-dioxin(TCDD) effected palatal organ development of fetal mouse.Methods:Kunming mice treated 40 ug/kg TCDD by lavage on day 12.5 of pregnancy were used as in the experimental group,isodose corn oil treated in the control group.On day 13.5,14.5 and 15.5 of pregnancy heads of the fetal mice were taken out and fixed.Conventional paraffin serial sections of palatal organ were preparated and dyed by hematoxylin-eosin,images of the palatal organs were collected and photoshop treated,3-D reconstruction of the palatal organ was performed by 3D-DOCTOR software.Results:3-D reconstruction images showed that palatal organs moved from on both sides above the tongue and gradually closed and merged in the control group.In the experimental group,the palatal organs moved from on both sides above the tongue was later than control group,gradually closed,but not merged,formed cleft palate.Conclusion:3D-DOCTOR software reconstruction can be used for the study of the development process effected by TCDD in the pregnant mouse.