鼻咽癌周围深层蔓延和预后与P53rn蛋白表达的相关性研究

摘要

目的研究鼻咽癌（NPC）的CT影像所表现的深层蔓延程度、预后与P53的关系，更全面的认识NPC的生物学特性。方法收集放疗前进行过CT检查并有详细临床记录的NPC活检标本70例，采用S-P法进行P53免疫组织化学染色，详细分析P53蛋白表达与NPC局部蔓延情况和临床预后的关系。结果本组NPC，P53阳性表达率68.57%，其与组织学类型和病理分级无关；Ⅱ型和Ⅲ型NPC的P53阳性率高于Ⅰ型NPC；P53的表达与有无颈淋巴结转移无关，但与转移颈淋巴结大小有关，即＜4 cm者的P53阳性率高于≥4 cm者；P53表达与NPC放疗后局部复发和远处转移无关。结论随肿瘤向鼻咽深层蔓延，P53阳性表达率增高，恶性程度加大，按CT影像所分3种类型，反映了NPC的生物学特性。%Objective　To investigate the correlation between degree of deeper infiltration of NPC on CT scan,prognosis and expression of P53 protein,in order to further recognize bilogical features of NPC.Methods　Collect 70 NPC biopsy specimens,which had performed CT scan before radiotherapy and had detailed clinical records.The expression of P53 protein was examined by means of S-P immunohistochemical technique.The correlation between expression of P53 protein and peripheral deeper infiltration and prognosis was analysed in detailed.Results　The results of these cases showed that the positive rate of over expression P53 were 68.57%,which had no relation with the histological types and pathological grades.The positive rate of P53 in Ⅱ、Ⅲ types had a higher positive rate than that of the Ⅰ type of NPC.Expression of P53 protein had no relation with neck lymph node metastasis,but had significant correlation with the size of metastatic lymph node,which is the positive rate of P53 in diameter of metastatic lymph node ＜4 cm was higher than that of diameter ≥4 cm.Expression of P53 had no relation with local recurrence and distant metastasis after radiotherapy.Conclusion　With the tumor infiltrating the deeper layer of nasopharynx,the positive rate of P53 expression is more and more high,and degree of malignant is increasing.The three types on CT scan represents the biological features of NPC.