曲古霉素A对人宫颈癌Hela细胞增殖及凋亡的影响

摘要

Objective:To investigate the effects of trichostatin A (TSA) on proliferation and apoptosis in human cervical carcinoma cell line Hela and the possible mechanism. Methods: Hela cells were treated with different concentration of histone deacetylase inhibitor TSA. Western blot was used to detect the acetyl level of histone H4 and real time PCR was used to detect mRNA of the p21 gene. Furthermore,cell growth was e-valuated by MTT assay and cell apoptosis rate was tested by flow cytometry. Results: After treated with TSA,the acetyl level of histone H4 and the expression of mRNA of the p21 gene both increased in Hela cells significantly(P<0. 05). Meanwhile,the cellular growth activity decreased and cell apoptosis increased(P< 0. 05). Conclusions:The TSA could effectively inhibit the cellular proliferation and induce apoptosis of cervical carcinoma cells by increasing the acetyl level of histone and by enhancing the p21 gene expression,and there are concentration dependent.%目的:探讨组蛋白乙酰化酶抑制剂曲古霉素A(TSA)对人宫颈癌Hela细胞增殖和调亡的作用及其机制.方法:不同浓度的TSA处理Hela细胞,应用Western blot法检测细胞内组蛋白H4(Ac-H4)乙酰化水平,荧光定量PCR方法检测p21 mRNA表达,MTT法检测细胞增殖,流式细胞仪检测细胞凋亡率.结果:随着TSA浓度增加,人宫颈癌Hela细胞内Ac-H4乙酰化水平增加(P＜0.05),p21 mRNA表达增加(P＜0.05),细胞增值率减少(P＜0.05),细胞凋亡率增加(P＜0.05).结论:TSA通过提高组蛋白乙酰化水平,增加p21基因表达,抑制人宫颈癌Hela细胞的增殖和诱导细胞凋亡,且呈浓度依赖性.