TAT-N24穿膜融合多肽对白血病细胞系HL60分化的影响

摘要

Objective To investigate the effect of cell-permeable fusion peptide TAT-N24 on the differentiation of acute myeloid leukemia HL60 cell line.Methods HL60 cells were incubated with TAT-N24.The cell morphological changes were observed before determination of the CD11b and CD14 expressions by flow cytometry.The proliferation ability of the HL60 cells was determined by BrdU/PI incorporation assay.Results The expressions of CD11b and CD14 were both elevated after the TAT-N24 treatment.The morphological features of HL60 cells tend to be like differentiated cells.Moreover,TAT-N24 could cooperate with all-trans retinoic acid in inducing the differentiation of HL60 cells.Meanwhile,the proliferation of HL60 cells could be inhibited by TAT-N24.Conclusions TAT-N24 could efficiently induce the differentiation of acute myeloid leukemia HL60 cells,and could inhibit the DNA synthesis of HL60 cells.TAT-N24 could also enhance the efficiency of all-trans retinoic acid in inducing HL60 cell differentiation.TAT-N24 may be potentially developed as an effective medicine for the treatment of the leukemia cell differentiation.%目的:观察TAT-N24穿膜融合多肽对急性髓系白血病细胞株HL60分化的影响.方法:TAT-N24作用于HL60细胞后,采用流式细胞术检测白血病分化指标CD11b及CD14,并观察HL60细胞经TAT-N24处理后的形态学变化,及BrdU/PI双掺入法测定DNA的合成.结果:HL60细胞在TAT-N24处理后,CD11b和CD14表达增高,且增高的趋势呈现TAT-N24浓度依赖性,同时形态学观察呈分化趋势,而且TAT-N24可以协同全反式维甲酸,增强其促进分化的作用.与此同时,BrdU/PI双掺入法显示TAT-N24使HL60细胞的增殖发生抑制.结论:TAT-N24穿膜融合多肽可促进急性髓系白血病细胞株HL60的分化,抑制其增殖,联合应用TAT-N24和全反式维甲酸具有明显的协同作用;TAT-N24有望被开发为有效的白血病分化治疗药物.