脑梗死和脑出血患者外周血中循环microRNA表达谱差异的初步分析

摘要

目的：利用microRNA芯片研究急性脑梗死和脑出血患者循环血浆中是否存在相关的miRNAs及有表达差异的miRNAs。方法：分别收集急性脑梗死、脑出血患者和健康对照者全血各3例，运用Norgen血浆外泌体RNA提取试剂盒提取血浆总RNA并通过定量PCR进行质量和浓度检测。采用μParaflorTM microRNA芯片技术进行miRNAs表达谱研究。用相关软件和统计学方法进行数据分析。结果：在脑梗死和脑出血患者血浆中发现8个与脑卒中相关的miRNAs，且有显著差异性表达（P＜0．05）。脑梗死和健康对照者、脑出血和健康对照者间分别有11、24个差异表达miRNAs，但两两比较上调或下调2倍以上的差异表达的miRNAs均无统计学意义（P＞0．05）。结论：脑梗死和脑出血组间miRNA的表达差异可能与不同类型脑卒中的发生发展相关，可为进一步研究急性脑卒中发生的表观遗传学分子机制提供依据。%Objective To explore the peripheral blood circulating microRNA expression profile of ischemic and hemorrhagic stroke patients by using microRNA array. Methods The whole blood of 3 patients with acute ischemic stroke, 3 patients with cerebral hemorrhage and 3 healthy volunteers were collected for whole blood RNA through Norgen exosome isolation kit, and then underwent quality and density measurement through PCR. The miRNAs expression profile was studied through μParaflorTM microRNA. All the data were analyzed by associated software and statistics methods. Results 8 miRNAs associated with cerebral stroke in ischemia stroke patients and cerebral hemorrhage patients were discovered, and showed significant difference (P<0.05). 11 differential expression miRNAs were found between ischemia stroke patients and control group, and 24 were found between hemorrhage patients and control group. However, after up-regulation, no significant difference was found (P > 0.05). Conclusions Different expression profiles of miRNA between ischemic stroke patients and hemorrhagic stroke patients may provide insights into their roles in the pathogenesis and progression of stroke. The study of miRNA can contributes to elucidate the epigenetics molecular mechanism of acute stroke.