目的：研究体外糖化终末产物（AGEs）对大鼠胰岛NADPH氧化酶表达的影响。方法：分离大鼠胰岛，用AGEs 刺激胰岛细胞2、12、24、48 h，采用实时荧光定量PCR检测Nox2表达水平的变化，化学发光法检测 NADPH 氧化酶活力。结果：AGEs 作用12、24 h 后，Nox2 mRNA 水平较对照组分别上升1．75、2．31倍（P＜0．05），随着AGEs作用时间的延长，NADPH 氧化酶活力逐渐升高，24 h 时达最高，后有所下降，但仍高于对照组。结论：糖化终末产物作用于胰岛后导致NADPH氧化酶活性增加，进而引起胰岛损害。%Objective To investigate the effects of advanced glycation end products (AGEs) on NADPH oxidase expression in rats′ pancreatic cells. Methods Isets of rats were isolated and intervened with AGEs for certain times (2, 12, 24, 48 h). The Nox2 mRNA expression was assayed by reverse transcription polymerase chain reaction (RT-PCR) and Real-time PCR. NADPH oxidase activity was detected by lucigenin-enhanced chemiluminescence. Results Nox2 mRNA level was 1.75, 2.31-fold higher in AGEs intervened group than control at 12 h and 24 h respectively. The NADPH oxidase activity was increased by AGEs stimulation in a time dependent manner. A distinct peak was at 24 hours and then with a decline , which is still higher than that of the control. Conclusion Exposure of rat islet to AGEs induces NADPH oxidase expression and leads to injury of islet.
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