目的：探讨中性粒细胞碱性磷酸酶（ NAP ）在急性髓系白血病（ AML ）患者中的表达情况及临床意义。方法：流式细胞术检测53例AML患者及50例正常对照组外周血NAP的表达水平；ELISA检测53例AML患者及50例正常对照组血浆中粒细胞-集落刺激因子（G-CSF）、中性粒细胞弹性蛋白酶（NE）、白细胞介素-8（IL-8）水平。结果：AML患者外周血NAP[1159（563～2033）AB／c]表达低于正常对照组[1862（1345～2382）AB／c]，差异有统计学意义（P＜0．05）；AML患者血浆中G-CSF、NE及IL-8[34．36（25．17～47．24） ng／L、397．56（324．63～563．84） pg／mL、585．34（403．61～866．54）pg／mL]表达低于正常对照组[54．52（46．27～77．14） ng／L、689．74（496．78～899．14）pg／mL、832．36（625．17～998．24） pg／mL]，差异有统计学意义（P＜0．05）。AML患者外周血NAP的水平与血清中G-CSF水平呈正相关（r＝0．621，P＝0．007）。结论：AML外周血中NAP表达显著降低，同时伴随血浆中性粒细胞相关因子 G-CSF、NE 及 IL-8分泌减少，推测 AML 患者中性粒细胞免疫功能受到抑制。%Objective To investigate the expression of NAP in patients with acute myeloid leukemia (AML). Methods Expression of NAP of peripheral blood was assessed by flow cytometry. The serum levels of G-CSF, NE and IL-18 in 53 patients with AML and 50 healthy controls were examined by ELISA. Results The expression of NAP in AML patients [1 159(563 ~ 2 033)AB/c] was decreased than that of healthy controls [1 862 (1 345 ~ 2 382)AB/c], (P < 0.05). The serum levels of G-CSF, NE and IL-8 from AML patients [34.36 (25.17 ~ 47.24) ng/L, 397.56 (324.63 ~ 563.84) pg/mL, 585.34 (403.61 ~ 866.54) pg/mL] were significantly lower than those of healthy controls[54.52(46.27 ~ 77.14) ng/L, 689.74(496.78 ~ 899.14) pg/mL, 832.36(625.17 ~ 998.24) pg/mL] (P < 0.05). The expression of NAP in AML patients was positively correlated with the serum level of G-CSF (r = 0.621, P = 0.007). Conclusions The decreased expression of NAP, G-CSF, NE and IL-8 in the peripheral blood with AML could speculated that neutrophils immune function in patients with AML was restrained.
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