目的:探讨表观治疗对非小细胞肺癌( NSCLC )细胞 DNMT1蛋白表达的影响及生物学作用。方法:NSCLC 细胞分组治疗,CCK-8、MTT 检测表观药物对 NSCLC 细胞的影响,Western blot 检测其对NSCLC细胞DNMT1蛋白表达的影响。结果:CCK-8和MTT结果显示,表观治疗可抑制 NSCLC 细胞的增殖及生长;Western blot 结果显示,5-氮杂-2′-脱氧胞苷和 MS-275的表观治疗可降低 DNMT1蛋白的表达。结论:5-氮杂-2′-脱氧胞苷和 MS-275的表观治疗能有效抑制 NSCLC 细胞中 DNMT1蛋白的表达,并且对NSCLC细胞的增殖和生长有明显抑制作用。%Objective To explore the characteristics and biological functions of epigenetic treatment to DNMT1 expression in NSCLC cell lines. Methods Lung cancer cells were divided into 4 groups , and drugs with different concentrations. The effect of epigenetic treatment on NSCLC cell line was detected by CCK-8 and MTT. The effect of epigenetic treatment on the expression of DNMT1 in NSCLC cell line was detected by Western blot. Results CCK-8 and MTT assay showed that treatment with 5-Aza-CdR and MS-275 inhibited the proliferation of NSCLC cells. Western blot showed that treatment with 5-Aza-CdR and MS-275 inhibited the DNMT1 expression in NSCLC cells. Conclusion The expression of DNMT1 gene in the NSCLC cell lines may be suppressed effectively by epigenetic treatment , and the gene may inhibit the proliferation and growth of NSCLC cell lines.
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