目的：探讨在宫颈病变的不同阶段，人乳头状瘤病毒（HPV）单独感染或多重感染的发生率，并探讨特定组合的HPV多重感染对宫颈癌发生风险的影响。方法：本研究共检测了102例女性宫颈标本，根据病变程度分为：低度鳞状上皮内病变／未见上皮病变或恶性病变组（LSIL／NILM组）与高度鳞状上皮内病变／浸润性宫颈癌组（ICC／HSIL组）。通过对样本进行PCR检测，鉴定HPV分型；然后进一步分析HPV多重感染的情况。通过计算ICC／HSIL或LSIL／NILM之间的优势比（OR），来检验特定HPV组合感染与宫颈癌病变之间的关系。结果：两组标本中，多重感染的发生率差异无显著性。与单一感染相比，HPV68和HPV16共同感染使患ICC／HSIL的风险增加。结论：高危型HPV病毒共同感染时，其ICC／HSIL发生的风险较单一高危型HPV病毒感染更高。%Objective To determine the incidence rate of HPV infection or multi-infections at different stages of cervical lesions in the development of cervical cancer , and the impact of specific types of HPV multi-infections on the risk of cervical cancer. Methods 103 samples of cervical tissues were detected and then divid-ed into ICC/HSIL group and LSIL/NILMF group according to the degree of pathological changes. HPV type was determined by PCR product sequencing. E6 nested multiplex PCR was performed to detect HPV multi-infections. Odds ratios were calculated to determinate the association between the sample category (LSIL/NILM or ICC/HSIL) and the specific types of HPV multi-infections. Results In HPV-positive samples, the rate of multi-in-fections had no significant differences between the two groups. Coinfection of HPV68 with HPV16 increased the risk of ICC/HSIL, as compared with HPV16 or HPV68 infection alone. Conclusions High-risk HPV coinfec-tions has a higher risk to induce ICC/HSIL than does HPV infection alone.
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