颗粒细胞端粒长度预测胚胎发育潜能及妊娠率的临床研究

摘要

Objective To investigate the relationship of relative telomere length in cumulus cells(CCs) with oocytes at different mature stages and the outcome of intracytoplasmic sperm injection and embryo transfer (ICSI-ET). Methods Oocyte-cumulus complex samples were collected from 92 patients undergoing ICSI-ET and patients were divided into group A including 55 women≤35 years and group B 37 women>35 years. The embryonic development ability and the result of clinical pregnancy were recorded in different groups. DNA was extracted from CCs and assessed for telomere length by real-time quantitative PCR. Results In ICSI-ET,the relative telomere length of CCs gradually shortened with the age.There was a significantly longer telomere length of CCs in the preg-nant subgroup than that in the non-pregnant subgroup in the two groups(P < 0.05). Conclusion The telomere length of CCs may be associated with the pregnancy outcome in ICSI-ET.%目的 研究行卵胞浆内单精子显微注射-胚胎移植(ICSI-ET)患者卵母细胞颗粒细胞的端粒长度与早期胚胎发育潜能及临床妊娠结局的相关性,为实验室评估胚胎发育潜能提供新的参考指标.方法 收集生殖中心92例行ICSI-ET患者的卵母细胞颗粒细胞,根据患者的年龄分为低龄(≤35岁)组55例和高龄(>35岁)组37例.利用QRT-PCR法检测患者的颗粒细胞端粒长度,分别记录每组患者卵裂率、优胚率及临床妊娠率.结果 卵母细胞颗粒细胞的端粒长度随着年龄的增加而降低(P<0.05).妊娠患者端粒长度较未妊娠患者长(P<0.05).结论 颗粒细胞端粒长度与早期胚胎发育潜能及临床妊娠率呈现一定的相关性.