补体C5基因多态性与肝硬化发病的关系研究

摘要

LAbstractJ Objective To evaluate the relationship of polymorphism of complement C5 gene and occurrence of liver cirrhosis in patients with chronic hepatitis B.Method Polymorphism of complement C5 gene rsl7611 was examined in 182 Chinese patients with chronic hepatitis B or liver cirrhosis by using TaqMan PCR genotyping method.Results Patients with genotypes G/G,A/G and A/A were detected with A/A genotype higher in patients with liver cirrhosis (P=0.022) ;By multivariate logistic regression analysis, genotype A/A was an independent factor associated with liver cirrhosis (odds ratio=5.1,P=0.008).Conclusion Polymorphism of complement C5 gene rsl7611 is associated with progression of liver disease in Chinese patients with hepatitis B virus infection.%目的 探讨补体C5基因多态性与肝硬化发生的关系.方法 慢性乙型肝炎和乙型肝炎肝硬化患者182例,提取白细胞DNA,采用Taqman实时PCR法进行C5基因rs17611位点的多态性基因型检测.结果在182例患者中,基因型为GG型者83例(45.6%),AG型者79例(43.4%),AA型者20例(11.0%);肝硬化患者C5基因rs17611位点AA基因型检出率较高;多元Logistic回归分析显示AA基因型是肝硬化发生的独立危险因子(OR=5.1,P=0.008).结论补体C5基因多态性可能与乙型肝炎病毒感染者发生肝硬化有关.