新跨膜蛋白非转移性黑色素瘤糖蛋白B在肾癌组织中的表达及意义

摘要

Objective:To investigate the role of glycoprotein non-metastatic melanoma protein B (GPNMB) in renal cell carcinoma (RCC).Methods:The method of immunohistochemistry (IHC) and Western blot were utilized to examine the expression of GPNMB in RCC and the normal adjacent tissues matched.Results:The expression of GPNMB was lower in RCC than in the matched normal adjacent tissues (P =0.022).Conclusion:The abnormal expression of GPNMB may play an important role in the development of RCC and the detection of GPNMB may be useful for the early diagnosis of tumor and its development.%目的:探讨非转移性黑色素瘤糖蛋白B(glycoprotein non-metastatic melanoma protein B,GPNMB)在肾癌组织中的表达及其临床意义.方法:应用组织芯片、免疫组织化学方法检测GPNMB在75例不同阶段肾癌及对应癌旁正常组织中的相对表达量,分析其表达量与肾癌患者年龄、性别、临床分期及病理分级的相关性；利用Western blot法检测GPNMB在肾癌组织及对应癌旁正常组织的表达情况.结果:GPNMB在肾癌组织表达水平低于癌旁正常肾组织,差异具有统计学意义(P=0.022)；Western blot结果显示,GPNMB在肾癌组织表达下调.结论:GPNMB在肾癌组织表达降低,可能参与肾癌的发生、发展及转归.