目的:研究深冷冻对人同种异体牙根免疫原性的影响.方法:制作人同种异体牙根粉,随机分为深冷冻与未深冷冻两组.用Mamar Blue法检测两种牙根粉与淋巴细胞共培养时对淋巴细胞生长增殖的影响,并进一步用ELISA法测定两种牙根粉刺激淋巴细胞产生人IFN-γ的情况.结果:Alamar Blue法检测到深冷冻组、未深冷冻组与淋巴细胞共培养对淋巴细胞增殖的影响无统计学意义(P＞0.05),ELISA法定量检测人IFN-γ表明未深冷冻组刺激淋巴细胞分泌IFN-γ的量高于深冷冻组.结论:人同种异体牙根免疫原性弱,而深冷冻法进一步降低了人同种异体牙根的免疫原性.%Objective:This study was designed to examine whether cryopreservation of human allogeneic tooth root are associated with immunogenicity. Methods: Human allograft root of tooth powder was made and randomly divided into 2 groups. Experiment group kept with cryopreservation, control group stored at 4℃ refrigerator. Human lymphocytes were obtained from 8 healthy donors. Both lymphocytes and root powders were grown in RPMI 1640 medium containing 10% fetal bovine serum at 37℃,5% CO2. Alamar Blue assay was used to observe the initial behavior of lymphocytes. Supernatant samples were used to determine activated IFN-7 with a IFN-7 kit. Results: There was no significant differences (P>0.05) on lymphocytes differentiation and proliferation between the two groups; ELISA confirmed that the protein levels of the activated IFN-7 in control group was higher than experiment group. Conclusion: The allogeneic tooth root has low immunogenicity, and cryopreservation method can further reduce the immunogenicity of allogeneic tooth root.
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