Chinese chestnut is one of the woody species of food which has been rapidly developed in China and has good prospects for development.Seedling grafting is an effective way for the large-scale production,and the key technology is the hypocotyl enlargement.The seeds of Castanea mollissima'yanshanzaofeng'were used as test materials,a randomized block design experiment was conducted.The seeds were macerated by chlorocholine chloride (CCC),garlic extract,and gibberellic acid (GA),and the water was used as the control.The treated seeds were then covered with different substrates,such as river sand,vermiculite and their mixtures with different thickness (5 cm,to 10 cm,15 cm).Effects of such treatments on the enlargement of bud-stock hypocotyls of Chinese chestnut were examined.The results indicated that 1)physical and chemical treatments could promote the enlargement of Chinese chestnut bud-stock hypocotyls,and physical treatment was superior to chemical treatment.2) With the chemical treatment,enlargement effect of GA was most significant,hypocotyls average roughness was 4.04 mm,coarse degree ＞ 4.00mm percentage was 49％.3) With the physical treatment,covering 10 cm of river sand was most significant,the hypocotyls average roughness was 4.44 mm,coarse degree ＞ 4.00 mm percentage was 78％.4)Most significant enlargement effects were achieved with the integrated physical and chemical treatments.Satisfactory results were obtained with the application of CCC and covering 10 cm of river sand,from which the average roughness was 4.54 mm,coarse degree ＞ 4.00 mm percentage was 80 ％.It was concluded that soaking the seeds with 3 000 mg · L-1 CCC,and then covering with 10cm river sand was the optimal method for the hypocotyl enlargement of chestnut seedlings.%板栗是我国具有发展前景的木本粮食树种之一.芽苗嫁接是板栗规模化生产的有效途径,其关键技术是胚轴增粗.以‘燕山早丰’种子为试验材料,采用随机区组试验设计,通过矮壮素、大蒜浸提液、赤霉素溶液浸种,清水为对照,浸种后覆盖不同成分(河沙、蛭石及其混合物)、厚度(5、10、15 cm)的基质,研究理化处理诱导板栗芽苗胚轴增粗的效应,以期探索板栗芽苗胚轴增粗的有效措施.结果表明:1)物理和化学处理均可促进板栗芽苗胚轴增粗,且物理处理优于化学处理.2)化学处理中,赤霉素的增粗效果最为明显,胚轴平均粗度为4.04 mm,粗度＞4.00 mm百分比为49％.3)物理处理中,覆盖10 cm河沙的增粗效果最明显,胚轴平均粗度为4.44 mm,粗度＞4.00mm百分比为78％.4)理化交互处理对胚轴的增粗作用更显著,其中矮壮素浸种十覆盖10 cm河沙的综合处理会得到最理想的增粗效果,胚轴平均粗度为4.54 mm,粗度＞4.00 mm百分比为80％.因此,采用河沙覆盖10 cm与3 000 mg·L-1矮壮素溶液浸种组合处理,诱导板栗芽苗胚轴增粗最理想.
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