Effects of BA, NAA, 2,4-D, and AgNO3 treatments on the callus induction and shoot regeneration from hypocotyl and cotyledon of sesame (Sesamum indicum L.).

摘要

The optimal conditions for callus induction and shoot regeneration from hypocotyls and cotyledons of sesame (S. indicum) were determined. After 4 weeks, the most effective concentrations of benzyladenine (BA) for callus induction (84.5-89.1% from the cotyledon and 85.9-89.6% from the hypocotyl) were 1 and 3 mg/litre, regardless of the concentrations of NAA. The AgNO3 concentrations most effective for callus induction (78.0-85.0% from the cotyledon and 79.0-85.6% from the hypocotyl) were 5, 10 and 15μM. Shoot regeneration from hypocotyls (25.8%) and cotyledons (10.0%) were highest in MS medium containing 3.0 mg BA/litre, 0.5 mg NAA/litre, 30 g sucrose/litre and 8 g agar/litre with AgNO3. Shoot regeneration from hypocotyls (20%) and cotyledons (8%)was promoted by AgNO3 treatment at 15μM. Treatments with 2,4-D were not effective for callus induction and shoot regeneration.