以西北农林科技大学选育的6个I－101（Poup lus alba）×84K（P．alba×P．glandulosa）优良杂种无性系（‘02－3－32’‘02－8－21’‘02－21－13’、秦白杨1号、秦白杨2号、秦白杨3号）、4个I－101×毛白杨优良杂种无性系（‘04－16－10’‘04－14－15’‘04－17－12’‘04－19－14’），以及I－101、84K、毛白杨30号3个对照无性系的1年生休眠枝条为试验材料，在一系列低温胁迫（－15、－20、－25、－30、－35℃）处理24 h后，测定他们的电导率、丙二醛（MDA ）质量摩尔浓度、可溶性蛋白质量分数、超氧化物歧化酶（ SOD ）活性等4个不同的生理生化指标变化，并通过隶属函数法对各无性系的抗寒性的结果进行比较与评价。结果表明：各无性系间抗寒性差异明显，抗寒性由强到弱表现为秦白杨2号、‘04－16－10’‘04－14－15’‘02－21－13’、秦白杨3号、84K、I－101、秦白杨1号、毛白杨30号、‘04－17－12’‘04－19－14’‘02－3－32’‘02－8－21’。研究表明，在4个抗寒指标综合评价中，秦白杨2号抗寒性表现最好；‘04－16－10’与‘04－14－15’抗寒性也明显强于其他无性系，可为抗寒性优良新品种选择及推广提供参考。%Taking annual dormancy branches of six hybrids of I-101 (Populus alba) ×84K (P.la ba×P.glandulosa), four hy-brids of I-101×P.tomentosa selected and bred by Northwest A&F University and three control clones (84K, I-101, P.to-mentosa NO.30) as experimental materials , we studied the changes of conductivity , the molality of MDA, the contents of soluble protein and SOD activities after the plants were treated under a series of low temperature (-15℃, -20 ℃, -25℃,-30℃ , and -35℃) for 24 h, and evaluated the differences of the clones in cold resistance by using membership function method .There were obvious differences among the clones of the ability of cold resistance .The descending order was Qin Bai yang NO.2,‘ 04-16-10’,‘04-14-15’, ‘02-21-13’, Qin Baiyang NO.3, 84K, I-101, Qin Baiyang NO.1, P.tomentosa NO.30,‘04-17-12’,‘04-19-14’, ‘02-3-32’, and ‘02-8-21’.NO.2 Qin Baiyang was the best in all the test results .‘04-16-10 ’ and ‘04-14-15 ’ clone materials had better cold resistance than others , which can provide reference for the popularization of new varieties with excellent cold resistance .
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