3种农药对天幕毛虫超氧化物歧化酶活性的影响

摘要

An experiment was conducted to study the effects of beta-cypermethrin, avermectin and phoxim on SODactivity of the 4th-instar larval Malacosoma neustria testacea through in vivo and in vitro tests by Nitrogen blue tetrazolium (NBT) test.Results showed that SOD activity was activated in vitro by 480 and 960 μg/L of beta-cypermethrin, but was inhibited by 1 920 and 3 840 μg/L of beta-cypermethrin. The in vivo SOD activity of M. neustria testacea larvae was activated by 480 μg/L of heta-cypermethrin, while the in vivo SOD activity was inhibited by 3 840 μg/L of beta-cypermethrin. The in vivo andin vitro SOD activities of Malacosoma neustria testacea larvae were all higher than that of the control, which were activated under 0 ～ 640 μg/L and 40 or 320 μg/L of avermectin treatment, respectively. The in vitro SOD activity of M. neustria testacea larvae was also higher than that of the control at 0 ～ 10 240 μg/L of phoxim. The activation was the strongest at 2 560 μg/L of phoxim and 33.46 percent higher than that of the control. In vivo, the SOD activity of M. neustria testacea .was higher than that of the control after 40 μg/L and 2 560 μg/L of phoxim treatment for 48 h, which were 207.78 percent and 287.46 percent higher than that of the control, respectively. These results indicate that SOD is involved in the response of M. neustria testacea larvae to beta-cypermethrin, avermectin and phoxim.%利用氮蓝四唑(NBT)法测定了高效氯氰菊酯、阿维菌素和辛硫磷对天幕毛虫4龄幼虫离体超氧化物歧化酶(SOD)和活体幼虫体内SOD活性的影响.结果表明:质量浓度为480、960μg/L的高效氯氰菊酯对离体SOD活性具有激活作用,质量浓度为1 920、3 840μg/L的高效氯氰菊酯表现抑制作用;质量浓度为480 μg/L的高效氯氰菊酯对活体幼虫体内SOD表现为激活作用,而质量浓度为3 840μg/L则表现为抑制作用.质量浓度为0～640μg/L的阿维菌素处理天幕毛虫幼虫离体SOD和质量浓度为40、320μg/L的阿维菌素处理天幕毛虫活体幼虫后,其SOD活性均高于对照,表现诱导增加作用.质量浓度为0～10 240μg/L的辛硫磷处理天幕毛虫幼虫,离体SOD活性均高于对照,且质量浓度为2 560μg/L的辛硫磷激活作用最强,比对照提高33.46%.质量浓度为40μg/L和2 560μg/L的辛硫磷处理天幕毛虫幼虫48 h,其活体幼虫体内SOD的活性也均高于对照,且处理48 h时间点的SOD活性激活作用最强,分别比对照提高207.78%和287.46%.研究结果表明,天幕毛虫幼虫SOD参与了3种农药作用响应,增强了体内自由基的清除能力.