欧美杂交杨 Pnd－LRR3基因克隆及其抗锈菌侵染表达

摘要

We studied the Pnd-LRR3 gene in the process of rust resistance function , cloned Pnd-LRR3 gene, and analyzed its bioinformatics to explore the molecular mechanism of the hybrid poplar Robusta ( Populus nigra×P.deltoides) and the role of resistance genes in the course of illness .By Q-PCR, the expression quantity of Pnd-LRR3 gene was highly increased when the virulent filial generation of Melampsora larici-populina E4 was inoculated for 6 h, while for 168 h got a significant negative correlation.Pnd-LRR3 gene could play a role in resistance of E4 infection.%为深入探究欧美杂交杨（Populus nigra×P．deltoides）感病的分子机理，以及抗病基因在发病过程中所起的作用，克隆了欧美杂交杨LRR3蛋白编码基因Pnd－LRR3。对Pnd－LRR3基因进行了生物信息学分析，并对其在抗锈菌过程中的功能进行了研究。 Q－PCR结果显示，强致病性的落叶松－杨栅锈菌（ Melampsora larici－populina） E4菌株接种6 h，Pnd－LRR3基因表达量上调增幅较大，168 h为显著下调。说明Pnd－LRR3基因在E4侵染过程中起到一定抗性作用，但最终无法阻止锈菌的侵染。