ISSR Analysis of M_1 Generation of Gladiolus hybridus Hort Treated by EMS

摘要

Gladiolus hybridus Hort is one of famous cutting flowers,being famous of big size,bright color,various shapes and long bloom period.New species should be cultivated in order to meet consumers' needs.Mutagenic breeding is a shortcut to cultivate new species of flowers.In this study,corm bud of G.hybridus Hort was treated with different concentrations of EMS.Then M 1 generation was analyzed by ISSR.Results showed that EMS was a very effective mutagenic agent for the corm bud of G.hybridus Hort.With the increase of EMS concentration,the mutagenic rate was increased,among which 1.0% EMS treatment had the biggest influence.However,with the same EMS concentration,there is no close relationship between the amount of mutagenic agent and the divergence of plants genomes,which offered a molecular basis for selecting plants with good mutation.