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Organic Compounds in Silica Bodies of Rice Revealed by X-ray Microanalysis and Micro-infrared Spectroscopy

机译:X射线微分析和红外光谱显示水稻二氧化硅体内的有机化合物

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To understand the possible mechanism controlling the formation of silicon bodies in higher plants, we need to know the nature of organic compounds associated with silicon bodies and induces silicon precipitation in plant cells. A new method was developed to isolate pure silicon body in fresh leaves of rice (Oryaa sativa L. Var. 297) at low temperature by grinding and centrifugation procedures, which avoided degradation of organic molecules induced by high temperature and strong oxidizing acids used in the traditional method. The energy dispersive X-ray spectrum under scanning electron microscope showed that there was a great amount of carbon in silicon bodies in addition to silicon and oxygen. Organic compounds intimately associated with silicon bodies were released by treatment with HF solution. Analysis of the organic compounds by micro-infrared spectroscopy revealed the presence of polyphenol and polysaccharide and a little protein.
机译:要了解控制高等植物中硅体形成的可能机制,我们需要了解与硅体相关的有机化合物的性质,并诱导植物细胞中硅的沉淀。开发了一种新的方法,该方法通过研磨和离心程序在低温下分离水稻新鲜叶片(Oryaa sativa L. Var。297)中的纯硅体,避免了高温和强氧化性酸诱导的有机分子降解。传统方法。扫描电子显微镜下的能量色散X射线光谱表明,除硅和氧外,硅体内还有大量碳。通过用HF溶液处理释放与硅体紧密结合的有机化合物。通过微红外光谱对有机化合物的分析表明存在多酚和多糖以及少量蛋白质。

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