首页> 中文期刊> 《东北农业大学学报:英文版 》 >RAPD analysis on the four kinds of color-flowered Salvia splendens Ker-Gawl

RAPD analysis on the four kinds of color-flowered Salvia splendens Ker-Gawl

             

摘要

In this study,we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl,and the optimal RAPD reaction conditions were the optimal reaction mixture(25μL total volume)that contained 2.0μL 10×buffer,0.45 mmol·L^(-1) dNTPs, 2.0 mmol·L^(-1)Mg^(2+),2 U Taq DNA polymerase,0.30 umol·L^(-1)primer and 40 ng genomic DNA.Total 84 bands were amplified from 12 primers used,and the differential bands had 28 bands,which was 33% of total bands.In cluster group analysis,the four kinds of color-flowered were divided into two styles.One style is that the red color and red-white color were grouped together,then they grouped with purple color into one cluster,and the white color was another style.

著录项

获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号